Recombinant Human UCK1, His-tagged

• Cat.No.: UCK1-199H
• Product Overview: UCK1, 143-273aa, Human, His tag, E.coli

Specification

• Description: UCK1 is a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. Recombinant human UCK1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 17.5 kDa(152aa)
• Protein length: 143-273aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MFYSQEIRDM FHLRLFVDTD SDVRLSRRVL RDVRRGRDLE QILTQYTTFV KPAFEEFCLP TKKYADVIIP RGVDNMVAIN LIVQHIQDIL NGDICKWHRG GSNGRSYKRT FSEPGDHPGM LTSGKRSHLE SS
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UCK1 uridine-cytidine kinase 1 [ Homo sapiens ]
• Official Symbol: UCK1
• Synonyms: UCK1; uridine-cytidine kinase 1; FLJ12255; URK1; UCK 1; uridine monophosphokinase 1; cytidine monophosphokinase 1; RP11-334J6.5
• Gene ID: 83549
• mRNA Refseq: NM_001135954
• Protein Refseq: NP_001129426
• MIM: 609328
• UniProt ID: Q9HA47
• Chromosome Location: 9q34.1
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem
• Function: ATP binding; kinase activity; nucleoside kinase activity; nucleotide binding; phosphotransferase activity, alcohol group as acceptor; transferase activity; uridine kinase activity