Recombinant Human Ubiquitin-Fold Modifier Conjugating Enzyme 1, His-tagged

• Cat.No.: UFC1-3534H
• Product Overview: Recombinant human UFC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (72-251aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. UFM1 is activated by a novel E1-like enzyme, UBA5, by forming a high-energy thioester bond. Activated UFM1 is then transferred to its cognate E2-like enzyme, UFC1, in a similar thioester linkage.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
• Molecular Mass: 21.6 kDa (187aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS-PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay )
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UFC1 ubiquitin-fold modifier conjugating enzyme 1 [ Homo sapiens ]
• Synonyms: UFC1; ubiquitin-fold modifier conjugating enzyme 1; HSPC155; Ufm1-conjugating enzyme1; ubiquitin-fold modifier-conjugating enzyme 1; CGI-126; Ubiquitin-fold modifier-conjugating enzyme 1
• Gene ID: 51506
• MIM: 610554
• mRNA Refseq: NM_016406
• Protein Refseq: NP_057490
• UniProt ID: Q9Y3C8
• Chromosome Location: 1q23.3
• Function: UFM1 conjugating enzyme activity; protein bindingTitle: Methionine sulfoxide reductases protect Ffh from oxidative damages in Escherichia coli.