Recombinant Human Ubiquitin-Conjugating Enzyme E2K, His-tagged

• Cat.No.: UBE2K-1790H
• Product Overview: Recombinant humanUBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Ubiquitin-conjugatingenzyme E2 K isoform 1, also known as UBE2K, is a member of theubiquitin-conjugating enzymes family. These enzymes participate in a varietyof cellular processes, including selective protein degradation, DNA repair,cell cycle control, and sporulation. In this mechanism, the ATP-coupledactivation and subsequent ligation of ubiquitin are catalyzed by separateenzymes functionally linked by ubiquitin carrier protein UBC1. Also, UBE2K isreportedly involved in Alzheimer"s disease, Huntington"s disease and proteinprocessing through its interaction with amyloid-?, huntingtin, and MHC-heavychain proteins.
• Concentration: 1 mg/ml
• Form: Suppliedas a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 10% glyceroland 50mM NaCl.
• Purity: > 95% by SDS -PAGE
• Sequence: 1-200 amino acids:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDENFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAMTLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGFDRNA
• Molecular Mass: 26.5 kDa(236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appearhigher).
• Applications: SDS-PAGE
• Storage: Storeat 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg Cor - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: UBE2K

Gene Information

• Gene Name: UBE2K ubiquitin-conjugatingenzyme E2K [ Homo sapiens ]
• Synonyms: UBE2K; ubiquitin-conjugating enzyme E2K; LIG; HIP2; HYPG; UBC1;E2-25K; DKFZp564C1216; DKFZp686J24237; HIP-2; E2(25K); OTTHUMP00000218439;OTTHUMP00000218440; OTTHUMP00000218441; OTTHUMP00000218442; ubiquitin-proteinligase; ubiquitin carrier protein; huntingtin interacting protein 2;huntingtin-interacting protein 2; ubiquitin-conjugating enzyme E2-25K;ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA;ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); NP_001104582.1; NP_001104583.1;NP_005330.1; EC 6.3.2.19
• Gene ID: 3093
• mRNA Refseq: NM_001111112
• Protein Refseq: NP_001104582
• MIM: 602846
• UniProt ID: C9JGP1
• Chromosome Location: 4p14
• Pathway: AdaptiveImmunity Signaling; Antigen processing; Class I MHC mediated antigenprocessing & presentation; Immune System; Innate Immunity Signaling;Negative regulators of RIG-I/MDA5 signaling; RIG-I/MDA5 mediated induction ofIFN-alpha/beta pathways; Ubiquitin mediated proteolysis
• Function: ATPbinding; acid-amino acid ligase activity; ligase activity; nucleotidebinding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity;ubiquitin-ubiquitin ligase activity