Recombinant Human UBE3A protein, His-tagged

• Cat.No.: UBE3A-1463H
• Product Overview: Recombinant Human UBE3A aa. (Ser158~Leu407) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser158~Leu407
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >92%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
  Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: UBE3A ubiquitin protein ligase E3A [ Homo sapiens (human) ]
• Official Symbol: UBE3A
• Synonyms: AS; ANCR; E6-AP; HPVE6A; EPVE6AP; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; renal carcinoma antigen NY-REN-54; ubiquitin-protein ligase E3A
• Gene ID: 7337
• mRNA Refseq: NM_000462.3
• Protein Refseq: NP_000453.2
• UniProt ID: Q05086