Recombinant Human UBE1 Protein, GST-tagged

• Cat.No.: UBE1-203H
• Product Overview: Recombinant Human UBE1 protein is produced by Sf21 insect cells expression system. This protein is fused with a GST tag.

Specification

• Species: Human
• Source: Insect cells
• Tag: GST
• Description: UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic Cys residue of UBE1 by formation of a thioester bond. The activated Ub is then passed on to a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
• Form: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol.
• Molecular Mass: 117.8 kDa
• Purity: > 95 % by SDS-PAGE
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: UBA1 ubiquitin like modifier activating enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: UBA1
• Synonyms: UBA1;A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X
• Gene ID: 7317
• mRNA Refseq: NM_003334
• Protein Refseq: NP_003325
• UniProt ID: P22314