Recombinant Human UBA7, His-tagged

• Cat.No.: UBA7-629H
• Product Overview: Recombinant Human UBA7 isproduced inE. coliand fused at the C-terminus to a His-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.
• Purity: >95%. Purified by Ni-affinity chromatography.
• Application: Activation of ISG15 for ISGylation of specific proteins in presence of dedicated E2 conjugating/E3 ligase enzymes. ISGylation pathway studies. Test proteins for ISG15 E3 ligase activity. Test proteins as potential targets for ISG15 modification.
• Storage: -80°C.

Gene Information

• Gene Name: UBA7 ubiquitin-like modifier activating enzyme 7 [ Homo sapiens ]
• Synonyms: UBA7; ubiquitin-like modifier activating enzyme 7; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-like; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; ubiquitin-like modifier-activating enzyme 7; D8; UBE2; UBA1B; UBE1L; MGC12713
• Gene ID: 7318
• mRNA Refseq: NM_003335
• Protein Refseq: NP_003326
• MIM: 191325
• UniProt ID: P41226
• Chromosome Location: 3p21
• Pathway: Parkinson"s disease; Ubiquitin mediated proteolysis
• Function: ATP binding; ISG15 activating enzyme activity; ligase activity; nucleotide binding