Species : |
Human |
Source : |
E.coli |
Tag : |
His&T7 |
Protein Length : |
Met1~Lys128 |
Description : |
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. |
Form : |
Freeze-dried powder |
Molecular Mass : |
Predicted Molecular Mass: 18.4kDa. |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
Purity : |
>90% |
Characteristic : |
The isoelectric point is 9.9. |
Applications : |
SDS-PAGE; WB; ELISA; IP. |
Stability : |
The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : |
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |