Recombinant Human Troponin T Type 2 (cardiac), His-tagged

• Cat.No.: TNNT2-6921H
• Product Overview: Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 36.4 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-285aa
• Description: TNNT2 is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Molecular Mass: 36.4 kDa (305aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TNNT2 troponin T type 2 (cardiac) [ Homo sapiens ]
• Official Symbol: TNNT2
• Synonyms: TNNT2; troponin T type 2 (cardiac); cardiomyopathy, hypertrophic 2 , CMH2, troponin T2, cardiac; troponin T, cardiac muscle; troponin T2, cardiac; cardiac muscle troponin T; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889
• Gene ID: 7139
• mRNA Refseq: NM_000364
• Protein Refseq: NP_000355
• MIM: 191045
• UniProt ID: P45379
• Chromosome Location: 1q32
• Pathway: Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; Muscle contraction, organism-specific biosystem
• Function: contributes_to ATPase activity; actin binding; tropomyosin binding; troponin C binding; troponin I binding