Recombinant Human TRMO Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TRMO-2888H
• Product Overview: C9orf156 MS Standard C13 and N15-labeled recombinant protein (NP_057565) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m6t6A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 48.6 kDa
• AA Sequence: MRGLEEPGPRPTATPCGCVKPALETGNLLTEPVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHKNGHLSCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIYLSGIDMIHGTPVLDIKPYIAEYDSPQNVMEPLADFNLQNNQHTPNTVSQSDSKTDSCDQRQLSGCDEPQPHHSTKRKPKCPEDRTSEENYLTHSDTARIQQAFPMHREIAVDFGLESRRDQSSSVAEEQIGPYCPEKSFSEKGTDKKLERVEGAAVLQGSRAETQPMAPHCPAGRADGAPRSVVPAWVTEAPVATLEVRFTPHAEMDLGQLSSQDVGQASFKYFQSAEEAKRAIEAVLSADPRSVYRRKLCQDRLFYFTVDIAHVTCWFGDGFAEVLRIKPASEPVHMTGPVGSLVSLGSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TRMO tRNA methyltransferase O [ Homo sapiens (human) ]
• Official Symbol: TRMO
• Synonyms: TRMO; tRNA methyltransferase O; NAP1; HSPC219; C9orf156; tRNA (adenine(37)-N6)-methyltransferase; Nef (lentivirus myristoylated factor) associated protein 1; Nef associated protein 1; nef-associated protein 1; thioesterase NAP1
• Gene ID: 51531
• mRNA Refseq: NM_016481
• Protein Refseq: NP_057565
• UniProt ID: Q9BU70