Recombinant human TRIP10, His-tagged

• Cat.No.: TRIP10-2477H
• Product Overview: Recombinant human TRIP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Cdc42-interacting protein 4, also known as TRIP10, belongs to the F-BAR family of proteins. These proteins contain an N-terminal, alpha-helical, region that is hydrophobic and considered to be related to the Bin Amphiphysin Rvs (BAR) protein family. Expressed in a variety of tissues, including kidney, brain, liver, lung, heart and pancreas, TRIP10 is required for the Insulin-dependent translocation of Glut4 to the plasma membrane and is essential for the coordination of membrane tubulation with Actin cytoskeletal reorganization during endocytosis.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 34.6kDa (309aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSDPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKKNKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMAEGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TRIP10 thyroid hormone receptor interactor 10 [ Homo sapiens ]
• Official Symbol: TRIP10
• Synonyms: TRIP10; thyroid hormone receptor interactor 10; salt tolerator , STOT; cdc42-interacting protein 4; Cdc42 interacting protein; CIP4; HSTP; STP; TRIP-10; protein Felic; salt tolerator; salt tolerant protein; TR-interacting protein 10; thyroid receptor interacting protein 10; thyroid receptor-interacting protein 10; STOT
• Gene ID: 9322
• mRNA Refseq: NM_004240
• Protein Refseq: NP_004231
• MIM: 604504
• UniProt ID: Q15642
• Chromosome Location: 19p13.3
• Pathway: Insulin Pathway, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Insulin-mediated glucose transport, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by Rho GTPases, organism-specific biosystem
• Function: identical protein binding; lipid binding