Recombinant Human transmembrane protein with EGF-like and two follistatin-like domains 1, His-tagged

• Cat.No.: TMEFF1-535H
• Product Overview: TMEFF1, 40-330aa, Human, His tag, E.coli

Specification

• Description: TMEFF1, also known as tomoregulin-1, is a type I transmembrane glycoprotein. This protein contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain of TMEFF1 can be released as a soluble protein. TMEFF1 is predominantly expressed in brain, but is downregulated in brain neoplasms. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto. Recombinant human TMEFF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol.
• Molecular Mass: 33.9 kDa (314aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSNQPPGG GGGSGGDCPG GKGKSINCSE LNVRESDVRV CDESSCKYGG VCKEDGDGLK CACQFQCHTN YIPVCGSNGD TYQNECFLRR AACKHQKEIT VIARGPCYSD NGSGSGEGEE EGSGAEVHRK HSKCGPCKYK AECDEDAENV GCVCNIDCSG YSFNPVCASD GSSYNNPCFV REASCIKQEQ IDIRHLGHCT DTDDTSLLGK KDDGLQYRPD VKDASDQRED VYIGNHMPCP ENLNGYCIHG KCEFIYSTQK ASCRCESGYT GQHCEKTDFS ILYVVPSRQK LTHV
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Protein length: 40-330 a.a.

Gene Information

• Gene Name: TMEFF1 transmembrane protein with EGF-like and two follistatin-like domains 1 [ Homo sapiens ]
• Official Symbol: TMEFF1
• Synonyms: TMEFF1; transmembrane protein with EGF-like and two follistatin-like domains 1; C9orf2; tomoregulin-1; cancer/testis antigen family 120; member 1; CT120.1; H7365; tomoregulin 1; cancer/testis antigen family 120, member 1; transmembrane protein with EGF-like and one follistatin-like domain; TR-1
• Gene ID: 8577
• mRNA Refseq: NM_003692
• Protein Refseq: NP_003683
• MIM: 603421
• UniProt ID: Q8IYR6
• Chromosome Location: 9q31