Recombinant Human TPST2 protein, His-tagged

• Cat.No.: TPST2-52H
• Product Overview: Recombinant human TPST2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: TPST2, also known as protein-tyrosine sulfotransferase 2, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. This protein is a type II integral membrane protein found in the Golgi body. Compared to TPST1, TPST2 has similar tissue distribution and overlapping substrate specificity. In contrast to TPST1-/- males with normal fertility in mice, TPST2-/- males are infertile due to severe defects in sperm motility.
• Source: Insect cells
• Species: Human
• Tag: His tag
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 40.4kDa (361aa)40-57KDa (SDS–PAGE under reducing conditions.)
• AA Sequence: ADPQQVLECR AVLAGLRSPR GAMRPEQEEL VMVGTNHVEY RYGKAMPLIF VGGVPRSGTT LMRAMLDAHP EVRCGEETRI IPRVLAMRQAWSKSGREKLR LDEAGVTDEV LDAAMQAFIL EVIAKHGEPA RVLCNKDPFT LKSSVYLSRL FPNSKFLLMV RDGRASVHSM ITRKVTIAGFDLSSYRDCLT KWNKAIEVMY AQCMEVGKEK CLPVYYEQLV LHPRRSLKLI LDFLGIAWSD AVLHHEDLIG KPGGVSLSKI ERSTDQVIKPVNLEALSKWT GHIPGDVVRD MAQIAPMLAQ LGYDPYANPP NYGNPDPFVI NNTQRVLKGD YKTPANLKGY FQVNQNSTSS HLGSSHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: TPST2 tyrosylprotein sulfotransferase 2 [ Homo sapiens ]
• Official Symbol: TPST2
• Synonyms: TPST2; tyrosylprotein sulfotransferase 2; TPST-2
• Gene ID: 8459
• mRNA Refseq: NM_001008566
• Protein Refseq: NP_001008566
• MIM: 603126
• UniProt ID: O60704
• Chromosome Location: 22q12.1
• Function: protein-tyrosine sulfotransferase activity; transferase activity