Recombinant Human TPP1 protein, His-tagged

• Cat.No.: TPP1-8094H
• Product Overview: Recombinant Human TPP1 aa. (Met1~Pro320) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1~Pro320
• Description: This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.
• Form: Freeze-dried powder
• Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.7.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TPP1 tripeptidyl peptidase 1 [ Homo sapiens (human) ]
• Official Symbol: TPP1
• Synonyms: CLN2; GIG1; LPIC; SCAR7; TPP-1; cell growth-inhibiting gene 1 protein; growth-inhibiting protein 1; lysosomal pepstatin; insensitive protease; tripeptidyl aminopeptidase; tripeptidyl peptidase I; tripeptidyl-peptidase 1
• Gene ID: 1200
• mRNA Refseq: NM_000391.3
• Protein Refseq: NP_000382.3
• UniProt ID: O14773