Recombinant Human TPBG protein, His-Avi-tagged, Biotinylated

• Cat.No.: TPBG-33H
• Product Overview: Biotinylated Recombinant Human TPBG protein(Q13641-1)(Ser32-Ser355), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser32-Ser355
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human TPBG, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TPBG trophoblast glycoprotein [ Homo sapiens ]
• Official Symbol: TPBG
• Synonyms: TPBG; trophoblast glycoprotein; 5T4; 5T4 AG; 5T4 oncofetal antigen; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein; M6P1; 5T4AG
• Gene ID: 7162
• mRNA Refseq: NM_001166392
• Protein Refseq: NP_001159864
• MIM: 190920
• UniProt ID: Q13641