Active Recombinant Human TNFSF18 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: TNFSF18-38H
• Product Overview: Biotinylated Human GITR Ligand, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 50-177 a.a.
• Predicted N Terminal: Gly
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human GITR, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human GITR Ligand, Fc Tag, Avi Tag with a linear range of 3.9-62.5 ug/mL.
• Molecular Mass: The protein has a calculated MW of 43.5 kDa. The protein migrates as 45-53 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF18 tumor necrosis factor (ligand) superfamily, member 18 [ Homo sapiens ]
• Official Symbol: TNFSF18
• Synonyms: TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237
• Gene ID: 8995
• mRNA Refseq: NM_005092
• Protein Refseq: NP_005083
• MIM: 603898
• UniProt ID: Q9UNG2