Recombinant Human TNFSF10 protein, His-Flag-tagged

• Cat.No.: TNFSF10-6855H
• Product Overview: Recombinant Human TNFSF10 protein(P50591-1)(Gly118-Gly281), fused with N-terminal His-Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: N-His-Flag
• ProteinLength: Gly118-Gly281
• Tag: N-His-Flag
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TRAIL R2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; APO2L; Apo-2L
• Gene ID: 8743
• mRNA Refseq: NM_001190942
• Protein Refseq: NP_001177871
• MIM: 603598
• UniProt ID: P50591