Recombinant Human TNFRSF1A, Fc-His

• Cat.No.: TNFRSF1A-1908H
• Product Overview: Recombinant Human Tumor Necrosis Factor Receptor Inhibitor produced inNSOis a disulfide linked homodimer, glycosylated, polypeptide chain. rHusTNFRI/Fc chimera (sTNFRI/Fc chimera) was fused to carboxy-terminal 6X his-tagged Fc part of human IgG1, via a linker peptide.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Description: Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated. TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses. It regulates immune and inflammatory responses.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.
• Molecular Weight: The predicted molecular weight of Recombinant Human TNF sRI is Mr 48 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
• Form: Purified.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.
• Dimers & Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHusTNFRI.
• Protein Content: Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of rHusTNFRI as a Reference Standard.
• Biological Activity: rHusTNFRI is fully biologically active when compared to standards.The activity was measured by its ability to neutralize apoptosis of mouse L929 cells treated with 0.25 ng/mL rhTNF-a. The ED50 for this effect is typically 0.4-1 ng/mL.
• Storage: -20°C (aliquot), avoid repeated freeze and thaw cycles.

Gene Information

• Gene Name: TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ]
• Synonyms: TNFRSF1A; tumor necrosis factor receptor superfamily, member 1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-RI; TNFR-I; TNF-R1; Tumor necrosis factor-binding protein 1; Tumor necrosis factor receptor superfamily member 1A, membrane form; CD120a antigen; tumor necrosis factor binding protein 1; tumor necrosis factor receptor type 1; tumor necrosis factor-alpha receptor
• Gene ID: 7132
• mRNA Refseq: NM_001065
• Protein Refseq: NP_001056
• MIM: 191190
• UniProt ID: P19438
• Chromosome Location: 12p13.2
• Pathway: Adipocytokine signaling pathway; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Cytokine-cytokine receptor interaction; MAPK signaling pathway; Apoptosis
• Function: protein complex binding; receptor activity; tumor necrosis factor receptor activity