Recombinant Human TIMP3 protein, His-tagged

• Cat.No.: TIMP3-852H
• Product Overview: Recombinant Human TIMP3(Cys24-Pro211) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 µm filtered PBS solution (pH 7.4).
• Molecular Mass: 23.4 kDa
• Purity: Purity: >90%, as determined by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining.
• Applications: ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc. Before use, heat the WBC control at 95 centigrade for 5 minutes. Spin down the sample and load 5 µL per lane onto SDS-PAGE gel.
• Storage: Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5~1.0 mg/mL with sterile deionized water or PBS.
• Protein length: Cys24-Pro211

Gene Information

• Gene Name: TIMP3 TIMP metallopeptidase inhibitor 3 [ Homo sapiens ]
• Official Symbol: TIMP3
• Synonyms: TIMP3; TIMP metallopeptidase inhibitor 3; SFD, tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory); metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3; SFD; K222; K222TA2; HSMRK222
• Gene ID: 7078
• mRNA Refseq: NM_000362
• Protein Refseq: NP_000353
• MIM: 188826
• UniProt ID: P35625
• Chromosome Location: 22q12.3
• Pathway: Endochondral Ossification, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem
• Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; protein binding

Reviews

09/05/2019

Manufacturers might offer customization options, such as different variants or modifications of the TIMP3 protein, to cater to specific research requirements.

01/28/2018

Such support helps researchers overcome challenges and obtain reliable results.

06/06/2016

This flexibility allows researchers to focus on specific aspects of TIMP3 protein functionality, regulation, or disease associations, enhancing the relevance and applicability of their work.