Recombinant Human Thiamine Triphosphatase, His-tagged

• Cat.No.: THTPA-786H
• Product Overview: Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 27.7 kDa (250aa) confirmed by MALDI-TOF(Real molecular weight on SDS-PAGE will be shift up).

Specification

• Cat. No.: THTPA-786H
• Description: THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8.5.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: THTPA thiamine triphosphatase [ Homo sapiens ]
• Synonyms: THTPA; thiamine triphosphatase; THTP; MGC2652; THTPASE; thiamine-triphosphatase; EC 3.6.1.28
• Gene ID: 79178
• mRNA Refseq: NM_001126339
• Protein Refseq: NP_001119811
• MIM: 611612
• UniProt ID: Q9BU02
• Chromosome Location: 14q11.2
• Pathway: Metabolic pathways; Thiamine metabolism
• Function: adenylate cyclase activity; hydrolase activity; thiamin-triphosphatase activity