Recombinant Human TGFB1(Leu30-Ser390) & GARP(His20-Leu628(Y137H)) Protein, His-Avi-tagged

• Cat.No.: TGFB1 & GARP-2196H
• Product Overview: Recombinant Human TGFB1(Leu30-Ser390) & GARP(His20-Leu628(Y137H)) Protein(Q14392(GARP(Y137H))&P01137(TGFB1)) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Leu30-Ser390 & His20-Leu628(Y137H)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GARP (Y137H) &Latent TGF Beta 1 Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 70.3 kDa (GARP(Y137H))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 71-75 kDa (GARP(Y137H))&13 kDa&42-45 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137