Recombinant Human TERT Protein, His-GST-tagged

• Cat.No.: TERT-438H
• Product Overview: Recombinant Human TERT Protein(Arg787~Arg1084), fused with N-terminal His and GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His-GST
• Protein length: Arg787~Arg1084
• Form: PBS, pH7.4, containing 0.01% SKL, 5%Trehalose.
• Bio-activity: Telomerase reverse transcriptase (TERT) is a subunit of the enzyme complex telomerase, which adds nucleotides to the ends of telomeres as they become shortened during cell division .Telomerase complex plays a key role in cancer formation by telomere dependent or independent mechanisms. According to statistics, human telomerase reverse transcriptase (h-TERT) is overexpressed in more than 85% of tumors with diverse histologies, with little expression in normal tissues. Expression of h-TERT correlates with activity of telomerase, which is required for the capacity for limitless replication, a hallmark of cancer. The X-Ray Repair Cross Complementing 6 (XRCC6) is high affinity receptor for TERT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TERT and recombinant rat XRCC6. Briefly, TERT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to XRCC6-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TERT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant TERT and recombinant rat XRCC6 was shown in Figure 1, the EC50 for this effect is 0.009 ug/mL.
• Molecular Mass: 63.4kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TERT telomerase reverse transcriptase [ Homo sapiens ]
• Official Symbol: TERT
• Synonyms: TERT; telomerase reverse transcriptase; EST2; hEST2; TCS1; TP2; TRT; telomerase catalytic subunit; telomerase-associated protein 2; hTRT; DKCA2; DKCB4
• Gene ID: 7015
• mRNA Refseq: NM_001193376
• Protein Refseq: NP_001180305
• UniProt ID: O14746