Recombinant Human TCF4 protein, His-tagged

• Cat.No.: TCF4-31H
• Product Overview: Recombinant human TCF4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: TCF4, as known as transcription factor 4 isoform I, is a protein acting as a transcription factor. This protein will bind to the immunoglobulin enhancer mu-E5/kappa-E2 motif. Also, it activates transcription by binding to the E-box (5 -CANNTG-3) found usually on SSTR2-INR, or somatostatin receptor 2 initiator element. This protein is found in the central nervous system, somites, and gonadal ridge during early development. Later in development it will be found in the thyroid, thymus, and kidneys while in adulthood it is found in lymphocytes, muscles, and gastrointestinal system.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 54.9kDa (513aa)
  35-100kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: MKFKQCRCSD TGLCCLDHEG KAEVYAPSAS TADYNRDSPG YPSSKPATST FPSSFFMQDG HHSSDPWSSS SGMNQPGYAG MLGNSSHIPQ SSSYCSLHPH ERLSYPSHSS ADINSSLPPM STFHRSGTNH YSTSSCTPPA NGTDSIMANR GSGAAGSSQT GDALGKALAS IYSPDHTNNS FSSNPSTPVG SPPSLSAGTA VWSRNGGQAS SSPNYEGPLH SLQSRIEDRL ERLDDAIHVL RNHAVGPSTA MPGGHGDMHG IIGPSHNGAM GGLGSGYGTG LLSANRHSLM VGTHREDGVA LRGSHSLLPN QVPVPQLPVQ SATSPDLNPP QDPYRGMPPG LQGQSVSSGS SEIKSDDEGD
  ENLQDTKSSE DKKLDDDKKD IKSITSNNDD EDLTPEQKAE REKERRMANN ARERLRVRDI NEAFKELGRM VQLHLKSDKP QTKLLILHQA VAVILSLEQQ VRERNLNPKA ACLKRREEEK VSSEPPPLSL AGPHPGMGDA SNHMGQMHHH HHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: TCF4 transcription factor 4 [ Homo sapiens ]
• Official Symbol: TCF4
• Synonyms: TCF4; transcription factor 4; bHLHb19; E2 2; ITF2; SEF2 1B; SL3-3 enhancer factor 2; E2-2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B
• Gene ID: 6925
• mRNA Refseq: NM_001083962
• Protein Refseq: NP_001077431
• MIM: 602272
• UniProt ID: P15884
• Chromosome Location: 18q21.1
• Pathway: CDO in myogenesis, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Myogenesis, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem
• Function: DNA binding; E-box binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; TFIIB-class binding transcription factor activity; TFIIB-class transcription factor binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; sequence-specific DNA binding RNA polymerase recruiting transcription factor activity; sequence-specific DNA binding transcription factor activity