Recombinant Human Suppressor Of Variegation 3-9 Homolog 1 (Drosophila), GST-tagged

• Cat.No.: SUV39H1-4891H
• Product Overview: Recombinant full-length human SUV39H1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: SUV39H1-4891H
• Description: SUV39H1 is a histone H3-specific methyltransferases that selectively methylates lysine-9 of the N terminus of histone H3 in vitro. SUV39H1 is also a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. SUV39H1 moves to the centromeres during mitosis where it functions as a histone methyltransferase, methylating Lys-9 of histone H3. SUV39H1 activity is regulated by acetylation at lysine residue 266 in its catalytic SET domain by SIRT1. SIRT1 interacts directly with, recruits, and deacetylates SUV39H1, and these activities independently contribute to elevated levels of SUV39H1 activity resulting in increased levels of the H3K9me3 modification.
• Applications: Western Blot
• Molecular Weight: 73 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Purity: > 70 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Lysine degradation

Gene Information

• Gene Name: SUV39H1 suppressor of variegation 3-9 homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: SUV39H1
• Synonyms: SUV39H1; suppressor of variegation 3-9 homolog 1 (Drosophila); MG44; KMT1A; SUV39H; suppressor of variegation 3-9 homolog 1; H3-K9-HMTase 1; Su(var)3-9 homolog 1; histone H3-K9 methyltransferase 1; histone-lysine N-methyltransferase, H3 lysine-9 specific 1; EC 2.1.1.43; OTTHUMP00000024298; Histone H3-K9 methyltransferase 1; Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1; suppressor of variegation 3-9 (Drosophila) homolog 1
• Gene ID: 6839
• mRNA Refseq: NM_003173
• Protein Refseq: NP_003164
• MIM: 300254
• UniProt ID: O43463
• Chromosome Location: Xp11.23
• Function: S-adenosylmethionine-dependent methyltransferase activity; chromatin binding; histone methyltransferase activity (H3-K9 specific); protein N-terminus binding; transferase activity; zinc ion binding