Recombinant Human SUMO2 Protein, Rhodamine 110 Labeled

• Cat.No.: SUMO2-05H
• Product Overview: This SUMO2 substrate is C-terminally derivatized with a bis-Glycine-Rhodamine-110 fluorophore. The bis-Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the rhodamine compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields an unparalleled signal-to-background. SUMO2-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 And SENP2, among other deSUMOylating enzymes. The substrate activity of SUMO2-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh-110.

Specification

• Description: SUMO1 is a Ubiquitin-like protein (UBL) that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO2 substrate is C-terminally derivatized with a bis-Gly-Rhodamine-110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the rhodamine compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields an unparalleled signal-to-background.
• Source: E. coli
• Species: Human
• Conjugation/Label: Rhodamine 110
• Molecular Mass: 11.03 kDa
• Purity: >97% by LCMS
• Applications: SUMO2-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, among other deSUMOylating enzymes. The substrate activity of SUMO2-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh-110. The Excitation and Emission of this substrate is 485nm and 535nM respectively.
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: Store at −80 centigrade after product arrival. Avoid multiple freeze/thaws. It is recommended to make multiple aliquots after the first thaw.
• Storage Buffer: 50mM Hepes pH 7.5, 100mM NaCl
• Concentration: 140uM, 1.5 mg/mL
• Detection Method: Fluorescence
• Substrate Properties: Protein-Based Substrate. Typical experimental concentration 50-500 nM.
• Excitation/Emission (nm): The Excitation and Emission of this substrate is 485nm and 535nm respectively.

Gene Information

• Gene Name: SUMO2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SUMO2
• Synonyms: SUMO2; SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae); SMT3 (suppressor of mif two 3, yeast) homolog 2 , SMT3 suppressor of mif two 3 homolog 2 (yeast) , SMT3H2; small ubiquitin-related modifier 2; SMT3B; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; small ubiquitin-like modifier 2; HSMT3; SUMO3; Smt3A; SMT3H2; MGC117191
• Gene ID: 6613
• mRNA Refseq: NM_001005849
• Protein Refseq: NP_001005849
• MIM: 603042
• UniProt ID: P61956