Recombinant Human SUMO1 Protein, Rhodamine 110 Labeled

• Cat.No.: SUMO1-04H
• Product Overview: This SUMO1 substrate is C-terminally derivatized with a Glycine-Rhodamine-110 (3,6-diamino-9-(2-carboxyphenyl)) fluorophore. The Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the Gly-Rh110 compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background. SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh110. The Excitation and Emission of this substrate is 485nm and 535nm respectively.

Specification

• Species: Human
• Source: E.coli
• Description: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO1 substrate is C-terminally derivatized with a bis-Gly-Rhodamine-110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the bis-Gly-Rh110 compound is hydrolyzed to mono-Gly-Rhodamine 110. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background.
• Conjugation/Label: Rhodamine 110
• Molecular Mass: 11.5 kDa
• Purity: >97% by LCMS
• Applications: SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched mono-Gly-Rh110. The Excitatino and Emission of this substrate is 485nm and 535nm respectively.
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: Store at −80 centigrade after product arrival. Avoid multiple freeze/thaws. It is recommended to make multiple aliquots after the first thaw.
• Storage Buffer: 50mM Hepes pH 7.5, 100mM NaCl
• Concentration: 160uM, 1.8 mg/mL
• Detection Method: Fluorescence
• Substrate Properties: Protein-Based Substrate. Typical experimental concentration 50-500 nM.
• Excitation/Emission (nm): The Excitation and Emission of this substrate is 485nm and 535nm respectively.

Gene Information

• Gene Name: SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SUMO1
• Synonyms: SUMO1; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast) , ubiquitin like 1 (sentrin) , UBL1; small ubiquitin-related modifier 1; GMP1; OFC10; PIC1; SMT3C; SMT3H3; SUMO 1; sentrin; SMT3 homolog 3; GAP modifying protein 1; ubiquitin-like protein UBL1; ubiquitin-like protein SMT3C; ubiquitin-homology domain protein PIC1; DAP1; SMT3; UBL1; SENP2
• Gene ID: 7341
• mRNA Refseq: NM_001005781
• Protein Refseq: NP_001005781
• MIM: 601912
• UniProt ID: P63165