Recombinant Human STRADA, His-tagged

• Cat.No.: STRADA-229H
• Product Overview: STRADA, 1-314aa, Human, His tag, E.coli

Specification

• Description: STE20-related kinase adapter protein alpha isoform 5, also known as STRADA, is a member of STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. STRADA-LKB1 interaction results in phosphorylation of STRADA and enhanced autophosphorylation of LKB1. Recombinant human STRADA protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 37 kDa (337aa)
• Protein length: 1-314aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSFLVSK PERIRTNDAS SESIASFSKQ EVMSSFLPEG GCYELLTVIG KGFEDLMTVN LARYKPTGEY VTVRRINLEA CSNEMVTFLQ GELHVSKLFN HPNIVPYRAT FIADNELWVV TSFMAYGSAK DLICTHFMDG MNELAIAYIL QGVLKALDYI HHMGYVHRSV KASHILISVD GKVYLSGLRS NLSMISHGQR QRVVHDFPKY SVKVLPWLSP EVLQQNLQGY DAKSDIYSVG ITACELANGH VPFKDMPATQ MLLEKLNGTV PCLLDTSTIP AEELTMSPSR SVANSGLSDS LTTSTPRPSN GPVPAPS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: STRADA STE20-related kinase adaptor alpha [ Homo sapiens ]
• Official Symbol: STRADA
• Synonyms: STRADA; STE20-related kinase adaptor alpha; STE20-related kinase adapter protein alpha; LYK5; NY BR 96; STE20 like pseudokinase; Stlk; STRAD; STRAD alpha; protein kinase LYK5; STE20-like pseudokinase; serologically defined breast cancer antigen NY-BR-96; PMSE; NY-BR-96; FLJ90524
• Gene ID: 92335
• mRNA Refseq: NM_001003786
• Protein Refseq: NP_001003786
• MIM: 608626
• UniProt ID: Q7RTN6
• Chromosome Location: 17q23.3
• Pathway: Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem; LKB1 signaling events, organism-specific biosystem; PI3K Cascade, organism-specific biosystem; PKB-mediated events, organism-specific biosystem
• Function: ATP binding; kinase binding; nucleotide binding; protein binding; protein kinase activator activity; NOT protein kinase activity; contributes_to protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups