Recombinant Human STC1, Flag-tagged

• Cat.No.: STC1-3745H
• Product Overview: Total 240 AA. MW: 25.9 kDa (calculated). C-Terminal Flag-tag, 10AA (highlighted). The AA sequence is identical to Swiss-Prot-P52823.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Description: Stanniocalcin-1 is a protein that in humans is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers.
• Form: Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 20mM NaCl, pH 7.5.
• Research topic: Bone and cartilage metabolism; Reproduction
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting; Cell culture and/or animal studies; ELISA
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: THEAEQNDSV SPRKSRVAAQ NSAEVVRCLN SALQVGCGAF ACLENSTCDT DGMYDICKSF LYSAAKFDTQ GKAFVKESLK CIANGVTSKV FLAIRRCSTF QRMIAEVQEE CYSKLNVCSI AKRNPEAITE VVQLPNHFSN RYYNRLVRSL LECDEDTVST IRDSLMEKIG PNMASLFHIL QTDHCAQTHP RADFNRRRTN EPQKLKVLLR NLRGEEDSPS HIKRTSHESA ASDYKDDDDK
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: STC1 stanniocalcin 1 [ Homo sapiens ]
• Official Symbol: STC1
• Synonyms: STC1; stanniocalcin 1; STC; stanniocalcin-1; STC-1; OTTHUMP00000123536; OTTHUMP00000225201; Stanniocalcin-1
• Gene ID: 6781
• mRNA Refseq: NM_003155
• Protein Refseq: NP_003146
• MIM: 601185
• UniProt ID: P52823
• Chromosome Location: 8p22-p12
• Function: hormone activity