Recombinant Human ST3 beta-galactoside alpha-2,3-sialyltransferase 5, His-tagged

• Cat.No.: ST3GAL5-335H
• Product Overview: ST3GAL5, 83-418aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 83-418 a.a.
• Description: Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. Recombinant human ST3GAL5 protein, fused to His-tag at N-terminus, was expressed in E.coli .
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 41.0 kDa (359aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLKLNYTT EECDMKKMHY VDPDHVKRAQ KYAQQVLQKE CRPKFAKTSM ALLFEHRYSV DLLPFVQKAP KDSEAESKYD PPFGFRKFSS KVQTLLELLP EHDLPEHLKA KTCRRCVVIG SGGILHGLEL GHTLNQFDVV IRLNSAPVEG YSEHVGNKTT IRMTYPEGAP LSDLEYYSND LFVAVLFKSV DFNWLQAMVK KETLPFWVRL FFWKQVAEKI PLQPKHFRIL NPVIIKETAF DILQYSEPQS RFWGRDKNVP TIGVIAVVLA THLCDEVSLA GFGYDLNQPR TPLHYFDSQC MAAMNFQTMH NVTTETKFLL KLVKEGVVKD LSGGIDREF
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ST3GAL5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 [ Homo sapiens ]
• Official Symbol: ST3GAL5
• Synonyms: ST3GAL5; ST3 beta-galactoside alpha-2,3-sialyltransferase 5; sialyltransferase 9 (CMP NeuAc:lactosylceramide alpha 2,3 sialyltransferase; GM3 synthase) , SIAT9; lactosylceramide alpha-2,3-sialyltransferase; SIATGM3S; ST3GalV; ST3Gal V; GM3 synthase; ganglioside GM3 synthase; alpha 2,3-sialyltransferase V; CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; sialyltransferase 9 (CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; GM3 synthase); SATI; SIAT9
• Gene ID: 8869
• mRNA Refseq: NM_001042437
• Protein Refseq: NP_001035902
• MIM: 604402
• UniProt ID: Q9UNP4
• Chromosome Location: 2p11.2
• Pathway: Ganglio Sphingolipid Metabolism, organism-specific biosystem; Glycosphingolipid biosynthesis - ganglio series, organism-specific biosystem; Glycosphingolipid biosynthesis - ganglio series, conserved biosystem; Glycosphingolipid biosynthesis, ganglio series, LacCer =>GT3, organism-specific biosystem; Glycosphingolipid biosynthesis, ganglio series, LacCer => GT3, conserved biosystem
• Function: lactosylceramide alpha-2,3-sialyltransferase activity; neolactotetraosylceramide alpha-2,3-sialyltransferase activity; sialyltransferase activity