Recombinant Human SSRP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SSRP1-1181H
• Product Overview: SSRP1 MS Standard C13 and N15-labeled recombinant protein (NP_003137) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 81.1 kDa
• AA Sequence: MAETLEFNDVYQEVKGSMNDGRLRL SRQGIIFKNSKTGKVDNIQAGELTE GIWRRVALGHGLKLLTKNGHVYKYD GFRESEFEKLSDFFKTHYRLELMEK DLCVKGWNWGTVKFGGQLLSFDIGD QPVFEIPLSNVSQCTTGKNEVTLEF HQNDDAEVSLMEVRFYVPPTQEDGV DPVEAFAQNVLSKADVIQATGDAIC IFRELQCLTPRGRYDIRIYPTFLHL HGKTFDYKIPYTTVLRLFLLPHKDQ RQMFFVISLDPPIKQGQTRYHFLIL LFSKDEDISLTLNMNEEEVEKRFEG RLTKNMSGSLYEMVSRVMKALVNRK ITVPGNFQGHSGAQCITCSYKASSG LLYPLERGFIYVHKPPVHIRFDEIS FVNFARGTTTTRSFDFEIETKQGTQ YTFSSIEREEYGKLFDFVNAKKLNI KNRGLKEGMNPSYDEYADSDEDQHD AYLERMKEEGKIREENANDSSDDSG EETDESFNPGEEEEDVAEEFDSNAS ASSSSNEGDSDRDEKKRKQLKKAKM AKDRKSRKKPVEVKKGKDPNAPKRP MSAYMLWLNASREKIKSDHPGISIT DLSKKAGEIWKGMSKEKKEEWDRKA EDARRDYEKAMKEYEGGRGESSKRD KSKKKKKVKVKMEKKSTPSRGSSSK SSSRQLSESFKSKEFVSSDESSSGE NKSKKKRRRSEDSEEEELASTPPSS EDSASGSDETRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SSRP1 structure specific recognition protein 1 [ Homo sapiens (human) ]
• Official Symbol: SSRP1
• Synonyms: SSRP1; structure specific recognition protein 1; FACT complex subunit SSRP1; facilitates chromatin remodeling 80 kDa subunit; FACT80; hSSRP1; FACTp80; cisplatin-DNA SSRP; FACT 80 kDa subunit; high mobility group box; structure-specific recognition protein 1; recombination signal sequence recognition protein 1; facilitates chromatin transcription complex subunit SSRP1; facilitates chromatin transcription complex 80 kDa subunit; chromatin-specific transcription elongation factor 80 kDa subunit; FACT; T160
• Gene ID: 6749
• mRNA Refseq: NM_003146
• Protein Refseq: NP_003137
• MIM: 604328
• UniProt ID: Q08945