Recombinant Human SPON1 protein, His-tagged

• Cat.No.: SPON1-02H
• Product Overview: Recombinant Human SPON1 protein, fused to His-tag, was expressed in HEK293.

Specification

• Description: SPON1, also known as F-Spondin, is a member of a sub-group of TSR(thrombospondin) molecules. It is a cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurities in vitro. This protein may contribute to the growth and guidance of axons. SPON1 is also major factor for vascular smooth muscle cell. It shows highest expression in lung and lowest expression in pancreas, skeletal muscle and ovary. This protein binds to the central extracellular of APP and inhibits beta-secretase cleavage of APP. Recombinant human SPON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol.
• Molecular Mass: 88.9 kDa
• Protein length: 29-807aa
• AA Sequence: FSDETLDKVP KSEGYCSRIL RAQGTRREGY TEFSLRVEGD PDFYKPGTSY RVTLSAAPPS YFRGFTLIAL RENREGDKEE DHAGTFQIID EEETQFMSNC PVAVTESTPR RRTRIQVFWI APPAGTGCVI LKASIVQKRI IYFQDEGSLT KKLCEQDSTF DGVTDKPILD CCACGTAKYR LTFYGNWSEK THPKDYPRRA NHWSAIIGGS HSKNYVLWEY GGYASEGVKQ VAELGSPVKM EEEIRQQSDE VLTVIKAKAQ WPAWQPLNVR AAPSAEFSVD RTRHLMSFLT MMGPSPDWNV GLSAEDLCTK ECGWVQKVVQ DLIPWDAGTD SGVTYESPNK PTIPQEKIRP LTSLDHPQSP FYDPEGGSIT QVARVVIERI ARKGEQCNIV PDNVDDIVAD LAPEEKDEDD TPETCIYSNW SPWSACSSST CDKGKRMRQR MLKAQLDLSV PCPDTQDFQP CMGPGCSDED GSTCTMSEWI TWSPCSISCG MGMRSRERYV KQFPEDGSVC TLPTEETEKC TVNEECSPSS CLMTEWGEWD ECSATCGMGM KKRHRMIKMN PADGSMCKAE TSQAEKCMMP ECHTIPCLLS PWSEWSDCSV TCGKGMRTRQ RMLKSLAELG DCNEDLEQVE KCMLPECPID CELTEWSQWS ECNKSCGKGH VIRTRMIQME PQFGGAPCPE TVQRKKCRIR KCLRNPSIQK LRWREARESR RSEQLKEESE GEQFPGCRMR PWTAWSECTK LCGGGIQERY MTVKKRFKSS QFTSCKDKKE IRACNVHPC
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90%
• Applications: SDS-PAGE
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: SPON1 spondin 1 [ Homo sapiens (human) ]
• Official Symbol: SPON1
• Synonyms: f-spondin; VSGP/F-spondin; spondin-1; SPON1; vascular smoth muscle cell growth-promoting factor
• Gene ID: 10418
• mRNA Refseq: NM_006108
• Protein Refseq: NP_006099
• MIM: 604989
• UniProt ID: Q9HCB6