Recombinant Human Spleen Tyrosine Kinase, GST-His

• Cat.No.: SYK-715H
• Product Overview: Recombinant Human SYK, Amino acids M1-N635, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 104,408 Da.

Specification

• Cat. No.: SYK-715H
• Description: SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells. It is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. In B cells, SYK plays a crucial role in intracellular signal transduction induced by oxidative stress as well as antigen receptor engagement. SYK has been shown to act as a potential tumor suppressor in breast cancer.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: SYK was confirmed as SYK by by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.094 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 20 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Protein length: 1-635 a.a.

Gene Information

• Gene Name: SYK spleen tyrosine kinase [ Homo sapiens ]
• Synonyms: SYK; spleen tyrosine kinase; FLJ25043; FLJ37489; DKFZp313N1010; OTTHUMP00000021628; OTTHUMP00000021629; EC 2.7.10.2; Tyrosine-protein kinase SYK
• Gene ID: 6850
• mRNA Refseq: NM_001135052
• Protein Refseq: NP_001128524
• MIM: 600085
• UniProt ID: P43405
• Chromosome Location: 9q22
• Pathway: B cell receptor signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Natural killer cell mediated cytotoxicity
• Function: ATP binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein domain specific binding; receptor signaling protein tyrosine kinase activity; transferase activity