Recombinant Human SOD2 protein, His-tagged

• Cat.No.: SOD2-355H
• Product Overview: Recombinant Human SOD2 protein (Lys25-Lys222), fused to His tag at C-terminus, was expressed in E.coli.

Specification

• Description: Superoxide dismutase [Mn], mitochondrial (SOD2),a member of the iron/manganese superoxide dismutase family, can form a homotetramer and bind one manganese ion per subunit. The expression of SOD2 is regulated by KRIT1. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), sporadic motor neuron disease, and cancer. Furthermore, Mn-SOD (SOD2) activity is essential to achieve optimal training-induced protection against both ischemia/reperfusion(IR)-induced cardiac arrhythmias and infarction.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 23.2 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 198
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SOD2
• Official Symbol: SOD2
• Synonyms: SOD2; superoxide dismutase 2, mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase; IPOB; MNSOD; MVCD6
• Gene ID: 6648
• mRNA Refseq: NM_000636
• Protein Refseq: NP_000627
• MIM: 147460
• UniProt ID: P04179