Recombinant Human SNRPE, His-tagged

• Cat.No.: SNRPE-3410H
• Product Overview: Recombinant human SNRPE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SNRPE, also known as SME, aids in the cytoplasmic construction of the UsnRNPs by binding to a conserved Sm site on UsnRNA and forming a stable snRNP core complex. As a core protein to UsnRNP, the SNRPE associates with the entire U family of snRNAs including U1–U6. SNRPE also interacts with DDX20 and Small nuclear ribonucleoprotein polypeptide F.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, and 1mM EDTA, 30% glycerol.
• Molecular Weight: 12.9 kDa (112aa) confirmed by MALDI-TOF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQV WLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKSRKQLGRIMLK GDN ITLL QSV SN.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Spliceosome; Transcription; Gene Expression; mRNA Processing; Metabolism of RNA

Gene Information

• Gene Name: SNRPE small nuclear ribonucleoprotein polypeptide E [ Homo sapiens ]
• Synonyms: small nuclear ribonucleoprotein polyp- eptide E;small nuclear ribonucleopr- otein E; snRNP-E; sm protein E; Sm protein E; SmE; SME; Sm-E; B-raf; SNRPE
• Gene ID: 6635
• mRNA Refseq: NM_003094
• Protein Refseq: NP_003085
• MIM: 128260
• UniProt ID: P62304
• Chromosome Location: 1q32
• Function: RNA binding; protein binding