Recombinant Human SNIP1, His-tagged

• Cat.No.: SNIP1-3537H
• Product Overview: Recombinant humanSNIP1 protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography techniques.

Specification

• Cat. No.: SNIP1-3537H
• Description: SNIP1is smad nuclear interacting protein contains a forkhead-associated (FHA)domain and acts as a nuclear inhibitor of CBP/p300. This protein is aninhibitor of the TGF-beta signal transduction pathway and to be important insuppressing transcriptional activation dependent on the co-activators CBP andp300. Inhibition of NF-kappa B activity is a function of the N-terminaldomain of SNIP1 and involves competition of SNIP1 and the NF-kappa B subunit,RelA/p65, for binding to p300, similar to the mechanism of inhibition of Smadsignaling by SNIP1.
• Application: SDS-PAGE
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl.
• Molecular Weight: 18.8 kDa (160aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHH SSGLVPRGSH MYIIDLGSGNGTFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDT SEIDRKDDED EEEEEEVSDSRWRLYPFKNDEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVF QYRLVEYTRA DGTVGRRVKP
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: SNIP1

Gene Information

• Gene Name: SNIP1 Smad nuclear interacting protein1 [ Homo sapiens ]
• Synonyms: SNIP1; Smad nuclear interacting protein1; FLJ12553; dJ423B22.2; RP3-423B22.3; smadnuclear-interacting protein 1; FHAdomain-containing protein SNIP1; Smadnuclear interacting protein (SNIP1); OTTHUMP00000004404
• Gene ID: 79753
• mRNA Refseq: NM_024700
• Protein Refseq: NP_078976
• MIM: 608241
• UniProt ID: Q8TAD8
• Chromosome Location: 1p34.3
• Pathway: Regulation of nuclear SMAD2/3signaling; TGF-beta Receptor Signaling Pathway
• Function: Protein binding