Recombinant Human SMPD1 protein, His & T7-tagged

• Cat.No.: SMPD1-6851H
• Product Overview: Recombinant Human SMPD1 aa. (His319~Pro579 (Accession # P17405)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• Protein length: His319~Pro579
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1 [ Homo sapiens (human) ]
• Official Symbol: SMPD1
• Synonyms: ASM; NPD; ASMASE; acid sphingomyelinase; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase
• Gene ID: 6609
• mRNA Refseq: NM_000543.4
• Protein Refseq: NP_000534.3
• UniProt ID: P17405