Recombinant Human SMO Protein, His-tagged

• Cat.No.: SMO-2707H
• Product Overview: Recombinant Human SMO Protein (Ala30-Ile234) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.9 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Ala30-Ile234
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SMO smoothened, frizzled family receptor [ Homo sapiens (human) ]
• Official Symbol: SMO
• Synonyms: SMO; smoothened, frizzled family receptor; SMOH, smoothened (Drosophila) homolog, smoothened homolog (Drosophila), smoothened, seven transmembrane spanning receptor; smoothened homolog; frizzled family member 11; FZD11; protein Gx; seven transmembrane helix receptor; smoothened, seven transmembrane spanning receptor; Gx; SMOH
• Gene ID: 6608
• mRNA Refseq: NM_005631
• Protein Refseq: NP_005622
• MIM: 601500
• UniProt ID: Q99835