Recombinant Human SMAD Family Member 3, GST-tagged

• Cat.No.: SMAD3-1094H
• Product Overview: Recombinant full-length human SMAD3 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 77 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Adipogenesis; Adherens junction; Alpha6-Beta4 Integrin Signaling Pathway; Cell cycle; Chagas disease (American trypanosomiasis); Colorectal cancer; EGFR1 Signaling Pathway; Endocytosis; HTLV-I infection; Pancreatic cancer; Pathways in cancer; Regulation of cytoplasmic and nuclear SMAD2/3 signaling; Regulation of nuclear SMAD2/3 signaling; Signaling by TGF beta; TGF Beta Signaling Pathway; Wnt signaling pathway

Gene Information

• Gene Name: SMAD3 SMAD family member 3 [ Homo sapiens ]
• Official Symbol: SMAD3
• Synonyms: SMAD3; SMAD family member 3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; MGC60396; DKFZp586N0721; DKFZp686J10186; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; OTTHUMP00000164305; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; MAD, mothers against decapentaplegic homolog 3 (Drosophila); SMAD, mothers against DPP homolog 3 (Drosophila); Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; Smad3
• Gene ID: 4088
• mRNA Refseq: NM_001145102
• Protein Refseq: NP_001138574
• MIM: 603109
• UniProt ID: P84022
• Chromosome Location: 15q21-q22
• Function: R-SMAD binding; co-SMAD binding; double-stranded DNA binding; protein binding; transcription factor binding; transforming growth factor beta receptor; ubiquitin protein ligase binding; contributes to DNA binding; phosphatase binding; sequence-specific DNA binding transcription factor activity; RNA polymerase II activating transcription factor binding; beta-catenin binding; core promoter proximal region sequence-specific DNA binding; transcription regulatory region DNA binding; ligand-regulated transcription factor activity