Recombinant Human SIRT3, His-tagged

• Cat.No.: SIRT3-30594TH
• Product Overview: Recombinant fragment of Human SIRT3 with N terminal His tag; 303aa, 33.5kDa.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
• Protein length: 282 amino acids
• Conjugation: HIS
• Molecular Weight: 33.500kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Widely expressed.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMSDKG KLSLQDVAELIRARACQRVVVMVGA GISTPSGIPDFRSPGSGLYSNLQQY DLPYPEAIFELPFFF HNPKPFFTLAKELYPGNYKPNVTHY FLRLLHDKGLLLRLYTQNIDGLERV SGIPASKLVEAHGTFASATCTVCQR PFPGEDIRADVMADRVPRCPVCTGV VKPDIVFFGEPLPQRFLLHVVDFPM ADLLLILGTSLEVEPFASLTEAVRS SVPRLLINRDLVGPLAWHPRSRDVA QLGDVVHGVESLVELLGWTEEMRDL VQRETGKLDGPDK
• Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin 3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2L3
• Gene ID: 23410
• mRNA Refseq: NM_001017524
• Protein Refseq: NP_001017524
• MIM: 604481
• Uniprot ID: Q9NTG7
• Chromosome Location: 11p15.5
• Pathway: Energy Metabolism, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; Signaling events mediated by HDAC Class III, organism-specific biosystem
• Function: NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; hydrolase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; metal ion binding