Recombinant Human SHBG Protein, His-tagged

• Cat.No.: SHBG-506H
• Product Overview: Recombinant Human SHBG Protein(Arg31~Ser401), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Arg31~Ser401
• Form: 10mM PBS, pH7.6, containing 5% trehalose.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Bio-activity: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein, a molecule that consists of a carbohydrate plus a protein, and is produced mainly in your liver. It binds to three sex hormones found in both men and women: estrogen, dihydrotestosterone (DHT) and testosterone. Its job is to transport these hormones throughout your blood to other tissues in your body. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and bsa-conjugated testosterone(Testo) . Briefly, biotin-linked recombinant human SHBG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to bsa-conjugated Testo -coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of SHBG and Testo was shown in Figure, and this effect was in a dose dependent manner.
• Molecular Mass: 42.0kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >99%

Gene Information

• Official Symbol: SHBG
• Synonyms: SHBG; sex hormone-binding globulin; ABP; androgen binding protein; MGC126834; MGC138391; TEBG; sex steroid-binding protein; testosterone-binding beta-globulin; testosterone-estrogen-binding globulin; testosterone-estradiol-binding globulin; testis-specific androgen-binding protein; SBP
• Gene ID: 6462
• mRNA Refseq: NM_001040
• Protein Refseq: NP_001031
• MIM: 182205
• UniProt ID: P04278