Recombinant Human SH3GL2, His-tagged

• Cat.No.: SH3GL2-15846H
• Product Overview: Recombinant human SH3GL2 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: SH3GL2 (SH3-domain GRB2-like 2), also known as endophilin-A1, belongs to the endophilin family. It contains a BAR domain and a SH3 domain. Members of the BAR domain protein superfamily are essential elements of cellular traffic. Endophilins are among the best studied BAR domain proteins. They have a prominent function in synaptic vesicle endocytosis (SVE), receptor trafficking and apoptosis, and in other processes that require remodeling of the membrane structure. SH3GL2 a novel tumor suppressor gene in laryngeal squamous cell carcinoma (LSCC), induces apoptosis of tumor cells by regulating intra-cellular signal transduction networks.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
• Molecular Mass: 42.5 kDa (376aa) confirmed by MALDI-TOF
• Protein length: 1-352aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSVAGL KKQFHKATQK VSEKVGGAEG TKLDDDFKEM ERKVDVTSRA VMEIMTKTIE YLQPNPASRA KLSMINTMSK IRGQEKGPGY PQAEALLAEA MLKFGRELGD DCNFGPALGE VGEAMRELSE VKDSLDIEVK QNFIDPLQNL HDKDLREIQHHLKKLEGRRL DFDYKKKRQG KIPDEELRQA LEKFDESKEI AESSMFNLLE MDIEQVSQLS ALVQAQLEYH KQAVQILQQV TVRLEERIRQ ASSQPRREYQ PKPRMSLEFP TGDSTQPNGG LSHTGTPKPS GVQMDQPCCR ALYDFEPENE GELGFKEGDI ITLTNQIDEN WYEGMLHGHSGFFPINYVEI LVALPH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SH3GL2 SH3-domain GRB2-like 2 [ Homo sapiens ]
• Official Symbol: SH3GL2
• Synonyms: SH3GL2; SH3-domain GRB2-like 2; endophilin-A1; CNSA2; EEN B1; SH3D2A; SH3P4; endophilin-1; SH3 domain protein 2A; Endophilin A1 BAR domain; bA335L15.1 (SH3-domain GRB2-like 2); SH3 domain-containing GRB2-like protein 2; EEN-B1; FLJ20276; FLJ25015
• Gene ID: 6456
• mRNA Refseq: NM_003026
• Protein Refseq: NP_003017
• MIM: 604465
• UniProt ID: Q99962
• Chromosome Location: 9p22
• Pathway: Axon guidance, organism-specific biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem
• Function: identical protein binding; lipid binding; protein binding