Recombinant Human Serpin Peptidase Inhibitor, Clade H (heat shock protein 47), Member 1, (collagen binding protein 1), His-tagged

• Cat.No.: SERPINH1-4718H
• Product Overview: Recombinant Human HSP47 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 439 amino acids (18-418 aa) and having a molecular mass of 48.9 kDa. HSP47 human recombinant is fused to 37 amino acid His Tag at N-terminus and purified by convential chromatogrpahy techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 18-418 a.a.
• Description: SERPINH1 is part of the serpin superfamily of serine proteinase inhibitors. SEPINH1 expression is induced by heat shock. HSP47 localizes to the endoplasmic reticulum lumen and binds collagen therefore it is a molecular chaperone which takes part in the maturation of collagen molecules, facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Autoantibodies to HSP47 protein have been found in rheumatoid arthritis. SERPINH1 binds specifically to collagen and acts as a chaperone in the biosynthetic pathway of collagen.
• Form: The SERPINH1 protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8 & 10% Glycerol.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) [ Homo sapiens ]
• Official Symbol: SERPINH1
• Synonyms: SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2; serpin H1; colligin-1; colligin-2; OTTHUMP00000229451; OTTHUMP00000229452; OTTHUMP00000229453; OTTHUMP00000229454; OTTHUMP00000229458; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)
• Gene ID: 871
• mRNA Refseq: NM_001207014
• Protein Refseq: NP_001193943
• MIM: 600943
• UniProt ID: P50454
• Chromosome Location: 11q13.5
• Function: collagen binding; serine-type endopeptidase inhibitor activity