Recombinant Human SEMA3E protein, His & T7-tagged

• Cat.No.: SEMA3E-1908H
• Product Overview: Recombinant Human SEMA3E aa. (Arg32~Phe516 (Accession # O15041)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Arg32~Phe516
• Description: Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.5

Gene Information

• Gene Name: SEMA3E semaphorin 3E [ Homo sapiens (human) ]
• Official Symbol: SEMA3E
• Synonyms: SEMA3E; semaphorin 3E; SEMAH; coll-5; M-SEMAH; M-SemaK; semaphorin-3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
• Gene ID: 9723
• mRNA Refseq: NM_001178129.1
• Protein Refseq: NP_001171600.1
• UniProt ID: O15041