Recombinant Human SAE1/SAE2 Protein, His-tagged

• Cat.No.: SAE1/SAE2-102H
• Product Overview: Recombinant Human SAE1/SAE2 protein is produced by Bacterial recombinant expression system. The SAE1 protein is fused with a His tag.

Specification

• Species: Human
• Source: Bacteria
• Tag: His
• Description: SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.
• Form: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol.
• Molecular Mass: SAE1 (33 kDa) & SAE2 (71.2 kDa)
• Purity: > 95 % by SDS-PAGE
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.
• Shipping: Dry ice shipping.

Gene Information

• Gene Name: SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens (human) ] & RBBP8 RB binding protein 8, endonuclease [ Homo sapiens (human) ]
• Official Symbol: SAE1 & RBBP8
• Synonyms: SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091) & SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)
• Gene ID: 10055 & 5932
• mRNA Refseq: NM_005500 & NM_002894
• Protein Refseq: NP_005491 & NP_002885
• UniProt ID: Q9UBE0 & Q99708