Recombinant Human RPN2, His-tagged

• Cat.No.: RPN2-7118H
• Product Overview: Recombinant human RPN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: RPN2, also known as ribophorin 2, is a dolichyl-diphosphooligosaccharide protein glycosyltransferase subunit 2. This protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. It is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 59.2kDa (539aa)
• Protein length: 23-540aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MLTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAAQASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIVEEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPVVVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIANTVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVAEPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: RPN2 ribophorin II [ Homo sapiens ]
• Official Symbol: RPN2
• Synonyms: RPN2; ribophorin II; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; RIBIIR; RPN II; RPNII; SWP1; ribophorin-2; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; RPN-II
• Gene ID: 6185
• mRNA Refseq: NM_001135771
• Protein Refseq: NP_001129243
• MIM: 180490
• UniProt ID: P04844
• Chromosome Location: 20q12-q13.1
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; Oligosaccharyltransferase, organism-specific biosystem
• Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; contributes_to dolichyl-diphosphooligosaccharide-protein glycotransferase activity; transferase activity