Recombinant Human RNF4 Protein (G120-I190), GST tagged

• Cat.No.: RNF4-0445H
• Product Overview: Recombinant Human GST-TEV-G-RNF4(G120-I190 end) Protein was expressed in E. coli.

Specification

• Description: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid
• Protein length: G120-I190
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: RNF4 ring finger protein 4 [ Homo sapiens (human) ]
• Official Symbol: RNF4
• Synonyms: RNF4; ring finger protein 4; SLX5; SNURF; RES4-26; E3 ubiquitin-protein ligase RNF4; E3 ubiquitin ligase RNF4; small nuclear RING finger protein; EC=6.3.2; Protein SNURF
• Gene ID: 6047
• mRNA Refseq: NM_002938
• Protein Refseq: NP_002929
• MIM: 602850
• UniProt ID: P78317