Recombinant Human Ring Finger Protein 181, His-tagged

• Cat.No.: RNF181-7149H
• Product Overview: Recombinant human RNF181 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 20.3 kDa.

Specification

• Description: E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 20.3 kDa (176aa) confirmed by MALDI-TOF
• Protein length: 1-153aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RNF181 ring finger protein 181 [ Homo sapiens ]
• Official Symbol: RNF181
• Synonyms: RNF181; ring finger protein 181; E3 ubiquitin-protein ligase RNF181; HSPC238
• Gene ID: 51255
• mRNA Refseq: NM_016494
• Protein Refseq: NP_057578
• MIM: 612490
• UniProt ID: Q9P0P0
• Chromosome Location: 2p11.2
• Function: ligase activity; metal ion binding; zinc ion binding