Recombinant Human Rho Family GTPase 3, His-tagged

• Cat.No.: RND3-2748H
• Product Overview: Recombinant human RND3 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =29.2kDa (261aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 0.1M NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RND3 Rho family GTPase 3 [ Homo sapiens ]
• Synonyms: RND3; Rho family GTPase 3; ARHE; Rho8; RhoE; memB; rho-related GTP-binding protein RhoE; protein MemB; OTTHUMP00000162685; OTTHUMP00000204380; OTTHUMP00000204381; small GTP binding protein Rho8; ras homolog gene family, member E; rho-related GTP-binding protein Rho8
• Gene ID: 390
• mRNA Refseq: NM_005168
• Protein Refseq: NP_005159
• MIM: 602924
• UniProt ID: P61587
• Chromosome Location: 2q23.3
• Function: GTP binding; GTPase activity; nucleotide binding