Recombinant Human RGMA protein, His-Avi-tagged, Biotinylated

• Cat.No.: RGMA-212H
• Product Overview: Biotinylated Recombinant Human RGMA protein(Q96B86-1)(Cys48-Gly422), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Cys48-Gly422
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: RGMA RGM domain family, member A [ Homo sapiens ]
• Official Symbol: RGMA
• Synonyms: RGMA; RGM domain family, member A; repulsive guidance molecule A; RGM
• Gene ID: 56963
• mRNA Refseq: NM_001166283
• Protein Refseq: NP_001159755
• MIM: 607362
• UniProt ID: Q96B86