Active Recombinant Human RFFL Protein, GST-tagged

• Cat.No.: RFFL-639H
• Product Overview: Recombinant Human RFFL fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: E3 ubiquitin-protein ligase RNF34L, also named CARP2 (Caspases-8/10 Associated RING protein 2), acts as RING‑domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34L also acts at the level of endocytic vesicles to limit the intensity of TNF-induced NF-κB activation by the regulated elimination of the adaptor protein RIP1.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Bio-activity: 18 nmol/min/mg
• Molecular Mass: 73 kDa
• Purity: >70%
• Applications: Ubiquitin Enzyme Assay, Western Blot
• Stability: 1 year at - 70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ml

Gene Information

• Gene Name: RFFL ring finger and FYVE-like domain containing E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: RFFL
• Synonyms: RFFL; ring finger and FYVE-like domain containing E3 ubiquitin protein ligase; ring finger and FYVE like domain containing; E3 ubiquitin-protein ligase rififylin; fring; rififylin; RNF34L; RNF189; RING finger protein 189; caspase regulator CARP2; FYVE-RING finger protein SAKURA; ring finger and FYVE-like domain containing 1; caspases-8 and -10-associated RING finger protein 2; RING finger and FYVE-like domain-containing protein 1; CARP2; FRING; CARP-2; RIFIFYLIN
• Gene ID: 117584
• mRNA Refseq: NM_001017368
• Protein Refseq: NP_001017368
• MIM: 609735
• UniProt ID: Q8WZ73