Recombinant Human Resistin

• Cat.No.: RETN-70H
• Product Overview: Recombinant Human Resistin is a 9.9 kDa protein containing 93 amino acid residues. It was expressed in E.coli. ASSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue- Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance.
• Specificity: The amino acid sequence of the Resistin Human Recombinant is homologous to the amino acid sequence of the Resistin Human without signal sequence.
• Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
• Purity: >98% (SDS-PAGE analyzed).
• Formulation: Recombinant Human Resistin was lyophilized from a concentrated (1 mg/ml) solution containg 0.03% NaHCO₃.
• Solubility: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
• Specific Activity: The biological activity was evidenced by the Resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10 ng/ml concentration.
• Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Gene Information

• Gene Name: RETN resistin [ Homo sapiens ]
• Synonyms: RETN; resistin; ADSF; RSTN; XCP1; MGC126603; MGC126609; found in inflammatory zone 3;C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; HXCP1; Adipose tissue-specific secretory factor; FIZZ3; RETN1; C/EBP-epsilon-regulated myeloid-specific secretedcysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3
• Gene ID: 56729
• mRNA Refseq: NM_020415
• Protein Refseq: NP_065148
• MIM: 605565
• UniProt ID: Q9HD89
• Chromosome Location: 19p13.2
• Function: hormone activity